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Pomeroyi and Rhodobacterales HTCC2255 genome comparison and continuing by adding each of the remaining 30 genomes one at a time. This relaxed ortholog definition was used because an all way RBH requirement was unworkable for the large number of genomes, each containing gene families represented by multiple members. We tested whether the order of the sequential best hit analysis resulted in substantial changes in the ortholog matrix or the outcome of the analyses (that is, by using a different order of adding genomes in the pair wise RBH), and found it did not.In addition to whole genome ortholog identification, a select group of ecologically relevant genes pathways was also identified using representative protein sequences of the target genes from a Roseobacter for which the gene functions had been experimentally verified.
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