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The first demonstration of a 3D film was in 1915. At Astor Theater in New York City, USA an audience wore red and green glasses for viewing for the first time 3D footage in film reels depicting dancing girls and Niagara Falls. The film was produced by Edwin Porter and William Waddell and utilized the anaglyph 3D process.

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Plates were then washed with PBS and blocked with 1% BSA for one hour at room temperature. Following this, one of the four anti antibodies was added at a concentration of 1 g/ml bFGF/147, 4 g/ml (Ab2), 0.5 g/ml (Ab3), or a 1:1000 dilution (DE6), incubated overnight at 4 and then employed for immunostaining. Further controls in the case of the bFGF/147 antibody included wells coated with the peptide used for immunization (amino acids 40 to 63 mapping within the amino terminal region of human FGF 2; Santa Cruz) at a concentration of 10 or 50 g/ml.

Most settlements were created by complete family groups with several generations often present in each settlement. Probably close to 80% of the families owned the land they lived and farmed on. They nearly all used English Common Law as their basic code of law and, except for the Dutch and Germans, spoke some dialect of English.

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